製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HELT
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse HELT.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse testis; Mouse heart; Human heart.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

画像

  • All lanes : Anti-HELT antibody (ab101842) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutesPlease note the current predicted molecular weight for this protein is 27kDa in Mouse and 36kDa in Human.

プロトコール

参考文献

This product has been referenced in:
  • Wende CZ  et al. Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis. PLoS One 10:e0127681 (2015). WB . Read more (PubMed: 25993409) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab101842.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録