Rabbit polyclonal to HELT
Rat, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse HELT.
This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse testis; Mouse heart; Human heart.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.
Information by UniProt
Hairy and enhancer of split-related protein HELT antibody
Western blot - Anti-HELT antibody (ab101842)
All lanes :
Anti-HELT antibody (ab101842) at 1 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Testis (Mouse) Tissue Lysate
Lane 3 :
Heart (Mouse) Tissue Lysate
Lane 4 :
Human heart tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
34 kDa (
why is the actual band size different from the predicted?
Exposure time :
8 minutesPlease note the current predicted molecular weight for this protein is 27kDa in Mouse and 36kDa in Human.
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