HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated (ab135395)
製品の概要
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製品名
HeLa Ser/Thr Phosphatase-Inhibited Whole Cell Lysate Set: Calyculin-Treated and Vehicle-Treated -
製品の概要
HeLa whole cell extracts were collected in lysis buffer after 3 hour treatment with Calyculin A (50 nM) or vehicle (DMSO) in serum-free media. The protein content was determined by BCA assay. Calyculin A is a potent phosphatase inhibitor. Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies.
Concentration:
HeLa Lysate - Vehicle Treated Control - 200 µg at 1 mg/mL
HeLa Lysate - Calyculin Treated - 200 µg at 1 mg/mL
The lysate is supplied in SDS-PAGE loading buffer with DTT and is ready to load on a gel.
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アプリケーション
適用あり: WB, SDS-PAGEmore details
製品の特性
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保存方法
Store at -80°C. Please refer to protocols. -
内容 2 units Calyculin-Treated HeLa Lysate 1 x 200µg Control for Calyculin-Treated HeLa Lysate 1 x 200µg -
研究分野
関連製品
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135395の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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SDS-PAGE |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
SDS-PAGE
Use at an assay dependent concentration. |
画像
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All lanes: Anti-Phosphoserine/Threonine antibody (ab17464) at 1/1000
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated Control at 20 µg
Secondary:
Goat polyclonal to Rabbit IgG – Pre-Adsorbed (HRP) at 1/6000 developed using the ECL technique.
Performed under reducing conditions. -
Lane 1: Marker
Lane 2: HeLa Whole Cell Lysate - Calyculin Treated at 20 µg
Lane 3: HeLa Whole Cell Lysate – Untreated control at 20 µg
Gel
10-20% acrylamide separation gel
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab135395 は論文での使用が確認できていません。