製品の概要
-
製品名HEK293 whole cell lysate
-
特記事項Cell line: HEK293 (Human embryonic kidney cell line).
Growth media: RPMI and 10% FBS (Fetal bovine serum).293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
-
アプリケーション適用あり: WBmore details
法規制情報
製品の特性
-
保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
-
バッファーpH: 6.80
Constituent: 5% Beta mercaptoethanol -
Concentration information loading... -
精製度Whole Cell Lysate
-
ライゼート 備考293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
-
研究分野
-
背景Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields.
アプリケーション
Our Abpromise guarantee covers the use of ab7902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent dilution. 293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
参考文献
ab7902 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"