HEK293 whole cell lysate (ab7902)

製品の概要

  • 製品名HEK293 whole cell lysate
    HEK293 ライゼート 製品一覧
  • 特記事項Cell line: HEK293 (Human embryonic kidney cell line).
    Growth media: RPMI and 10% FBS (Fetal bovine serum).


    293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 精製度Whole Cell Lysate
  • ライゼート 備考293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野
  • 背景Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields.

アプリケーション

Our Abpromise guarantee covers the use of ab7902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.
293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

HEK293 whole cell lysate (ab7902) 使用論文

ab7902 has not yet been referenced specifically in any publications.

Product Wall

We do supply the positive control for the Anti-S6K1 (phospho T229) antibody (ab5231).


The positive control is HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902). Please refer to the Cell Lysate datasheet for further info...

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Thank you for contacting us.

HEK293 cells can also be used as positive control.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. I am sorry for the confusion. I was unsure whether you wanted replacement product or a credit note/refund. I have issued credit notes/refunds for these products. Your credit note ID for AB93922 is xxx. Your credit ...

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Thank you for sending this information.

If you would like, I can issue free of charge replacements of the antibody and lysate with another anti-ThPok antibody such as ab23388 or ab70991 and our recommended positive control lysate. Like ab9...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to off...

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Thank you for your telephone inquiry this afternoon. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody in western blotting.

I do hope the use of a new secondary antibody will resolve this ...

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Thank you for contacting us. I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates ca...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Unfortunately we do not sell the positive control employed as a positive control for ab22510; HEK293 cells overexpressing human FOXP3. Howev...

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I'm sorry but we do not currently stock RIPA buffer.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"