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Our Abpromise guarantee covers the use of ab20896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. ab20896 is a lysate produced from 293 cells transfected with a 55 kDa HA-tagged minigene. Pretreatment 10 min in 95 degree cersius is recommended before loading on SDS-PAGE for Western blotting. 10 µg per lane is recommended for mini gels.|
ab20896 has not yet been referenced specifically in any publications.