製品の概要

  • 製品名Anti-HEF1 (phospho S369) antibody
    HEF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HEF1 (phospho S369)
  • 特異性ab110854 reacts with Human HEF1 phosphoprotein.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, a wide range of other species
  • 免疫原

    Synthetic peptide corresponding to Human HEF1 (internal sequence) (phospho S369).
    Database link: Q14511

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/1000.

ターゲット情報

  • 機能Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
  • 組織特異性Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.
  • 配列類似性Belongs to the CAS family.
    Contains 1 SH3 domain.
  • ドメインContains a central domain containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif confers specific interaction with the HLH proteins ID2, E12 and E47. It is absolutely required for the induction of pseudohyphal growth in yeast and mediates homodimerization and heterodimerization with p130cas.
    The SH3 domain interacts with two proline-rich regions of focal adhesion kinase.
  • 翻訳後修飾Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.
    Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor in rabbit. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton.
  • 細胞内局在Cytoplasm > cytoskeleton > spindle and Cytoplasm > cell cortex. Nucleus. Golgi apparatus. Cell projection > lamellipodium. Cytoplasm. Cell junction > focal adhesion. Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cas like docking antibody
    • Cas scaffolding protein family member 2 antibody
    • CAS-L antibody
    • CAS2 antibody
    • CasL antibody
    • CASL_HUMAN antibody
    • CASS2 antibody
    • Crk associated substrate related antibody
    • Crk associated substrate related protein antibody
    • CRK-associated substrate-related protein antibody
    • dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)) antibody
    • dJ49G10.2 antibody
    • dJ761I2.1 (enhancer of filamentation (HEF1)) antibody
    • dJ761I2.1 antibody
    • Enhancer of filamentation 1 antibody
    • Enhancer of filamentation 1 p55 antibody
    • HEF 1 antibody
    • HEF1 antibody
    • NEDD-9 antibody
    • Nedd9 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 9 antibody
    • P105 antibody
    • Renal carcinoma antigen NY-REN-12 antibody
    see all

Anti-HEF1 (phospho S369) antibody (ab110854) 使用論文

ab110854 has not yet been referenced specifically in any publications.

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