Anti-NEDL1 抗体 (ab121264)
Key features and details
- Rabbit polyclonal to NEDL1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NEDL1 antibody -
製品の詳細
Rabbit polyclonal to NEDL1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human NEDL1 aa 500-614.
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ポジティブ・コントロール
- Human cerebral cortex tissue; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fix cells with PFA/Triton X-100.
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特記事項 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fix cells with PFA/Triton X-100. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS. -
組織特異性
Predominantly expressed in neurons of adult and fetal brain. Weakly expressed in the kidney. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 23072 Human
- Omim: 610384 Human
- SwissProt: Q76N89 Human
- Unigene: 164453 Human
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別名
- C2 and WW domain-containing protein 1 antibody
- E3 ubiquitin-protein ligase HECW1 antibody
- HECT antibody
see all
画像
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ab121264, at 1/15 dilution, staining NEDL1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong nuclear and cytoplasmic positivity in neuronal cells.
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ab121264, at 4 µg/ml, staining NEDL1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence, showing positivity in nucleus but not nucleoli & cytoplasm.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab121264 は 2 報の論文で使用されています。
- Wang C et al. Integrating HECW1 expression into the clinical indicators exhibits high accuracy in assessing the prognosis of patients with clear cell renal cell carcinoma. BMC Cancer 21:890 (2021). PubMed: 34348693
- Pan H et al. Novel somatic mutations identified by whole-exome sequencing in muscle-invasive transitional cell carcinoma of the bladder. Oncol Lett 11:1486-1492 (2016). PubMed: 26893765