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Synthetic peptide within residues: FHPEELKDVI VGNTDYDWKT FEKNARYEPG YNSSHPTIVM FWKAFHKLTL , corresponding to internal sequence amino acids 900-949 of Human HECT E3 ubiquitin ligase (NP_057407).
Our Abpromise guarantee covers the use of ab83853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab83853 has not yet been referenced specifically in any publications.