製品の概要

  • 製品名Anti-HEC1 antibody [EPR5342]
    HEC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5342] to HEC1
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human HEC1.

  • ポジティブ・コントロール
    • Jurkat, HeLa, NCCIT, and Human spleen lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • 配列類似性Belongs to the NDC80/HEC1 family.
    • 発生段階Expression peaks in mitosis.
    • 翻訳後修飾Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
    • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • 参照データベース
    • 別名
      • from Entrez Gene ; antibody
      • HEC antibody
      • Highly expressed in cancer antibody
      • Highly expressed in cancer protein antibody
      • Highly expressed in cancer rich in leucine heptad repeats antibody
      • HsHec1 antibody
      • hsNDC80 antibody
      • Kinetochore associated 2 antibody
      • Kinetochore associated protein 2 antibody
      • Kinetochore protein Hec1 antibody
      • Kinetochore protein NDC80 homolog antibody
      • Kinetochore-associated protein 2 antibody
      • KNTC2 antibody
      • ndc80 antibody
      • NDC80 homolog kinetochore complex component antibody
      • NDC80 kinetochore complex component homolog antibody
      • NDC80, S. cerevisiae, homolog of antibody
      • NDC80_HUMAN antibody
      • Retinoblastoma associated protein HEC antibody
      • Retinoblastoma-associated protein HEC antibody
      • TID3 antibody
      see all

    Anti-HEC1 antibody [EPR5342] 画像

    • All lanes : Anti-HEC1 antibody [EPR5342] (ab109496) at 1/10000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : NCCIT lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 74 kDa

    Anti-HEC1 antibody [EPR5342] (ab109496) 使用論文

    ab109496 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109496.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"