製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to HEC1
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: YEKKATLIKSSEE, corresponding to C terminal amino acids 630-642 of Human HEC1.

  • ポジティブ・コントロール
    • Hela lysate.
  • 特記事項
    GenBank Accession Number – NP_006092 Gene Ontology terms - mitotic chromosome segregation; mitosis; centromere; nucleus

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 74 kDa).

    This antibody does not seem suitable for using in ICC/IF (please refer to Abreview).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
    • 配列類似性
      Belongs to the NDC80/HEC1 family.
    • 発生段階
      Expression peaks in mitosis.
    • 翻訳後修飾
      Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
    • 細胞内局在
      Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
    • Information by UniProt
    • 参照データベース
    • 別名
      • from Entrez Gene ; antibody
      • HEC antibody
      • Highly expressed in cancer antibody
      • Highly expressed in cancer protein antibody
      • Highly expressed in cancer rich in leucine heptad repeats antibody
      • HsHec1 antibody
      • hsNDC80 antibody
      • Kinetochore associated 2 antibody
      • Kinetochore associated protein 2 antibody
      • Kinetochore protein Hec1 antibody
      • Kinetochore protein NDC80 homolog antibody
      • Kinetochore-associated protein 2 antibody
      • KNTC2 antibody
      • ndc80 antibody
      • NDC80 homolog kinetochore complex component antibody
      • NDC80 kinetochore complex component homolog antibody
      • NDC80, S. cerevisiae, homolog of antibody
      • NDC80_HUMAN antibody
      • Retinoblastoma associated protein HEC antibody
      • Retinoblastoma-associated protein HEC antibody
      • TID3 antibody
      see all

    画像



    • Predicted band size : 74 kDa


      ab3393 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    プロトコール

    参考文献

    ab3393 has not yet been referenced specifically in any publications.

    レビューと Q&A

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Dec 15 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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