Application
Western blot
Sample
Human Tissue lysate - whole (heart atria)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
heart atria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: GE (Amersham) ECL Mouse IgG, HRP-Linked F(ab')2 Fr
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Sheep
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
West Pico (Pierce)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 223 kDa
Additional data
Additional Notes
1: human atrial extract
2: mouse HL1 cardiac myocyte cell line
3: primary neonatal rat cardiac myocyte
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Paul Townsend
Verified customer
投稿 Apr 21 2008