Anti-heavy chain Myosin/MYH3 抗体 (ab124205)
Key features and details
- Rabbit polyclonal to heavy chain Myosin/MYH3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-heavy chain Myosin/MYH3 antibody
heavy chain Myosin/MYH3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to heavy chain Myosin/MYH3 -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab124205 are tested in IHC-P. Previous batches were additionally validated in WB. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab264038.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Chicken, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human heavy chain Myosin/MYH3 aa 100-200 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates. IHC: Mouse Skeletal Muscle.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124205の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
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IHC-P |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Muscle contraction. -
関連疾患
Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic. -
配列類似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
発生段階
Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle. -
ドメイン
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
細胞内局在
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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参照データベース
- Entrez Gene: 417309 Chicken
- Entrez Gene: 489504 Dog
- Entrez Gene: 4621 Human
- Entrez Gene: 17883 Mouse
- Entrez Gene: 24583 Rat
- Omim: 160720 Human
- SwissProt: P02565 Chicken
- SwissProt: P11055 Human
see all -
別名
- embryonic antibody
- fast skeletal muscle antibody
- HEMHC antibody
see all
画像
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All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
IHC image of Anti-heavy chain Myosin/MYH3 antibody staining in a section of formalin-fixed paraffin-embedded mouse skeletal muscle performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab124205, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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All lanes : Anti-heavy chain Myosin/MYH3 antibody (ab124205) at 1 µg/ml
Lane 1 : Skeletal Muscle (Human) Tissue Lysate - fetal normal tissue
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lane 5 : Heart (Human) Tissue Lysate - fetal normal tissue
Lane 6 : Human skin tissue lysate - total protein (ab30166)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Additional bands at: 190 kDa, 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab124205 は 17 報の論文で使用されています。
- Riederer I et al. Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection. PLoS Negl Trop Dis 16:e0010166 (2022). PubMed: 35171909
- Lamin V et al. microRNA-29a Regulates ADAM12 Through Direct Interaction With ADAM12 mRNA and Modulates Postischemic Perfusion Recovery. J Am Heart Assoc 11:e025727 (2022). PubMed: 35946473
- Matsushita Y et al. The fate of early perichondrial cells in developing bones. Nat Commun 13:7319 (2022). PubMed: 36443296
- Ling X et al. Lidocaine Inhibits Myoblast Cell Migration and Myogenic Differentiation Through Activation of the Notch Pathway. Drug Des Devel Ther 15:927-936 (2021). PubMed: 33688167
- Yu JA et al. LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B. Cell Death Discov 7:73 (2021). PubMed: 33837177