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A synthetic peptide corresponding to residues in Human HE4
This product is a recombinant rabbit monoclonal antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Our Abpromise guarantee covers the use of ab109298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 13 kDa).|
|Flow Cyt||1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
Immunocytochemistry/Immunofluorescence analysis of SK-OV-3 (human ovarian adenocarcinoma) cells labelling HE4 with ab109298 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The negative control was SK-OV-3 cells stained with only DAPI, without ab109298.
Confocal image showing cytoplasmic staining on SK-OV-3 cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"