製品の概要

  • 製品名Anti-HE4 antibody
    HE4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HE4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.

  • ポジティブ・コントロール
    • Normal human epididymis or human ovarian tumour.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
  • 配列類似性Contains 2 WAP domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 5 isoforms produced by alternative splicing.
  • 別名
    • dJ461P17.6 antibody
    • EDDM4 antibody
    • epididymal protein 4 antibody
    • Epididymal secretory protein E4 antibody
    • Epididymis specific whey acidic protein type four disulfide core antibody
    • HE 4 antibody
    • Major epididymis specific protein E4 antibody
    • Major epididymis-specific protein E4 antibody
    • MGC57529 antibody
    • Putative protease inhibitor WAP5 antibody
    • WAP 5 antibody
    • WAP domain containing protein HE4 antibody
    • WAP domain containing protein HE4 V4 antibody
    • WAP four disulfide core domain 2 antibody
    • WAP four disulfide core domain protein 2 antibody
    • WAP four-disulfide core domain protein 2 antibody
    • WAP5 antibody
    • WFDC 2 antibody
    • WFDC2 antibody
    • WFDC2_HUMAN antibody
    see all

Anti-HE4 antibody 画像

  • ab24480 (4µg/ml) staining HE4 in human lung using an automated system (DAKO Autostainer Plus).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-HE4 antibody (ab24480) 使用論文

This product has been referenced in:
  • Markovic-Lipkovski J  et al. Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis. PLoS One 10:e0137028 (2015). IHC-P ; Human . Read more (PubMed: 26327314) »
  • Tokuishi K  et al. Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma. Tumour Biol 33:103-9 (2012). WB, IHC-P ; Human . Read more (PubMed: 22015929) »

See all 4 Publications for this product

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Thank you for your enquiry. The concentration of this lot is 30 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. The antibody is a polyclonal that was raised against a GST-fusion of the full length HE4 protein. As a result, it is likely to recognize all isoforms, but this has not been specifically tested. I hope that this inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"