製品の概要

  • 製品名
    Anti-HDPTP antibody
  • 製品の詳細
    Rabbit polyclonal to HDPTP
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide of Human HDPTP.

    Synthetic peptide of Human HDPTP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HDPTP transfected 293T whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    WB: 1/500. Detects a band of approximately 75 kDa in transfected lysate (predicted molecular weight: 179 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.
    • 配列類似性
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
      Contains 1 BRO1 domain.
      Contains 2 TPR repeats.
      Contains 1 tyrosine-protein phosphatase domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus. Cytoplasm. Cytoplasmic vesicle. Endosome. Cytoplasm > cytoskeleton > cilium basal body.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZP564F0923 antibody
      • EC 3.1.3.48 antibody
      • HD PTP antibody
      • HD-PTP antibody
      • His domain containing protein tyrosine phosphatase antibody
      • His domain protein tyrosine phosphatase antibody
      • His domain-containing protein tyrosine phosphatase antibody
      • KIAA1471 antibody
      • Protein tyrosine phosphatase non receptor type 23 antibody
      • Protein tyrosine phosphatase TD 14 antibody
      • Protein tyrosine phosphatase TD14 antibody
      • PTN23_HUMAN antibody
      • PTP TD14 antibody
      • PTP-TD14 antibody
      • PTPN 23 antibody
      • PTPN23 antibody
      • Tyrosine protein phosphatase non receptor type 23 antibody
      • Tyrosine-protein phosphatase non-receptor type 23 antibody
      see all

    画像

    • ICC/IF image of ab12553 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12553 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-HDPTP antibody (ab12553) at 1 µg/ml + PTPN23 293T cell transient overexpression lysate (denatured) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 179 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

    参考文献

    ab12553 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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