Anti-HDLBP 抗体 [EPR5267] (ab109324)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5267] to HDLBP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HDLBP antibody [EPR5267]
HDLBP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5267] to HDLBP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: 293T, Jurkat, HeLa and HepG2 cell lysates IHC-P: Human stomach tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5267 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109324の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol. -
配列類似性
Contains 14 KH domains. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3069 Human
- Entrez Gene: 110611 Mouse
- Entrez Gene: 64474 Rat
- Omim: 142695 Human
- SwissProt: Q00341 Human
- SwissProt: Q8VDJ3 Mouse
- SwissProt: Q9Z1A6 Rat
- Unigene: 471851 Human
see all -
別名
- HBP antibody
- HDL binding protein antibody
- HDL-binding protein antibody
see all
画像
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All lanes : Anti-HDLBP antibody [EPR5267] (ab109324) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 141 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted? -
ab109324, at a 1/100 dilution, staining HDLBP in paraffin embedded Human stomach tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109324 は 2 報の論文で使用されています。
- Zinnall U et al. HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Nat Commun 13:2727 (2022). PubMed: 35585045
- Mobin MB et al. The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation. Nat Commun 7:12848 (2016). PubMed: 27665711