Mouse monoclonal [26C4a] to HDGF
Recombinant fragment of human HDGF, from an internal sequence.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Protein G purified
ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 21960592
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
Belongs to the HDGF family.
Contains 1 PWWP domain.
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
Information by UniProt
Western blot - Anti-HDGF antibody [26C4a] (ab58644)
Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein
at 25 µg
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
The immunogen shown in the WB is tagged.
This product has been referenced in:
et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011).
Read more (PubMed: 21960592) »
et al. Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Cell Death Dis 2:e224 (2011).
Read more (PubMed: 22048167) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"