製品の概要

  • 製品名Anti-HDC2 / PHC2 antibody
    HDC2 / PHC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDC2 / PHC2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 553-835 of Human HDC2/ PHC2 (BC029269; UniProt ID: Q8IXK0).

  • ポジティブ・コントロール
    • HEK293 and MCF7 lysates

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab124405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 36, 30 kDa (predicted molecular weight: 91,88,36,30 kDa).

ターゲット情報

  • 機能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 配列類似性Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメインHD1 motif interacts with SAM domain of PHC1.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 5 isoforms produced by alternative splicing.
  • 別名
    • EDR2 antibody
    • early development regulator 2 (homolog of polyhomeotic 2) antibody
    • early development regulator 2 like antibody
    • Early development regulatory protein 2 antibody
    • hPH2 antibody
    • MGC163502 antibody
    • mph2 antibody
    • PH2 antibody
    • PHC2 antibody
    • PHC2_HUMAN antibody
    • polyhomeotic 2 antibody
    • polyhomeotic like 2 antibody
    • Polyhomeotic-like protein 2 antibody
    see all

Anti-HDC2 / PHC2 antibody 画像

  • All lanes : Anti-HDC2 / PHC2 antibody (ab124405) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : MCF7 cell lysate


    Predicted band size : 91,88,36,30 kDa
    Observed band size : 30,36 kDa (why is the actual band size different from the predicted?)

Anti-HDC2 / PHC2 antibody (ab124405) 使用論文

ab124405 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 50 µg
Treatment 2i and LIF
Specification Mouse embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
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投稿 Sep 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"