製品の概要

  • 製品名Anti-HDAC9 antibody [EPR5223]
    HDAC9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5223] to HDAC9
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of Human HDAC9.

  • ポジティブ・コントロール
    • K562, HepG2, and Raji cell lysates; Human colon tissue; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-HDAC9 antibody (Alexa Fluor® 488) [EPR5223] (ab206713)

    Anti-HDAC9 antibody (Alexa Fluor® 647) [EPR5223] (ab207050)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
      Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
    • 組織特異性Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
    • 関連疾患Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
    • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
    • 翻訳後修飾Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
      Sumoylated.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HD 7 antibody
      • HD 7B antibody
      • HD 9 antibody
      • HD7 antibody
      • HD7B antibody
      • HD9 antibody
      • HDAC 7 antibody
      • HDAC 7B antibody
      • HDAC 9 antibody
      • HDAC 9B antibody
      • HDAC 9FL antibody
      • HDAC antibody
      • HDAC7 antibody
      • HDAC7B antibody
      • HDAC9 antibody
      • HDAC9_HUMAN antibody
      • HDAC9B antibody
      • HDAC9FL antibody
      • HDRP antibody
      • Histone deacetylase 4/5 related protein antibody
      • Histone deacetylase 7 antibody
      • Histone deacetylase 7B antibody
      • Histone deacetylase 9 antibody
      • Histone deacetylase 9A antibody
      • Histone deacetylase related protein antibody
      • Histone deacetylase-related protein antibody
      • KIAA0744 antibody
      • MEF2 interacting transcription repressor MITR antibody
      • MEF2 interacting transcription repressor protein antibody
      • MEF2-interacting transcription repressor MITR antibody
      • MITR antibody
      see all

    Anti-HDAC9 antibody [EPR5223] 画像

    • All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 111 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.

    Anti-HDAC9 antibody [EPR5223] (ab109446) 使用論文

    This product has been referenced in:
    • Sebastian S  et al. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes Dev 27:1247-59 (2013). Read more (PubMed: 23723416) »
    • Klinz FJ  et al. Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo. Histochem Cell Biol : (2012). ICC/IF, IHC-FrFl ; Human . Read more (PubMed: 22297573) »

    See all 2 Publications for this product

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