製品の概要

  • 製品名Anti-HDAC9 antibody
    HDAC9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC9
  • 特異性ab53102 detects endogenous levelss of total HDAC9 protein.
  • アプリケーション適用あり: ELISA, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from human HDAC9.

  • ポジティブ・コントロール
    • WB: HepG2 cell extract This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • 組織特異性Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • 関連疾患Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 翻訳後修飾Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

Anti-HDAC9 antibody 画像

  • ICC/IF image of ab53102 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53102 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-HDAC9 antibody (ab53102) at 1/300 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with peptide


    Predicted band size : 117 kDa
    Observed band size : 117 kDa

Anti-HDAC9 antibody (ab53102) 使用論文

This product has been referenced in:
  • Milde T  et al. HDAC5 and HDAC9 in medulloblastoma: novel markers for risk stratification and role in tumor cell growth. Clin Cancer Res 16:3240-52 (2010). WB ; Human . Read more (PubMed: 20413433) »

See 1 Publication for this product

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The amino acid range of this immunogen is between aa 1000-1100 of human human HDAC9 isoforms 5, 6, and 7 with ACC# Q9UKV0-5, Q9UKV0-6, and Q9UKV0-7. This a...

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