製品の概要

  • 製品名Anti-HDAC9 antibody
    HDAC9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC9
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Rat, Hamster, Human, Chinese Hamster
  • 免疫原

    Synthetic peptide:

    EVPVGLEPISPLDLRT

    (Human).

  • ポジティブ・コントロール
    • Mouse intestine tissue lysate for Western blot.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 110-140 kDa. A ~75 kDa and a ~60 kDa unknown bands can also be detected in some tissue lysates.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • 組織特異性Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • 関連疾患Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 翻訳後修飾Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

Anti-HDAC9 antibody 画像

  • Anti-HDAC9 antibody (ab18970) at 2.5 µg/ml + mouse intestine tissue lysate 50ug

    Observed band size : 110-140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab18970 staining human breast cancer cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% BSA for 1 hour and 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 3 hours at 25°C.  A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab18970 staining HDAC9 in human neuroblastoma by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol then blocked using 1% BSA for 2 hours. Samples were then incubated with primary antibody at 1/200 for 16 hours at 18°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used undiluted.

    See Abreview

Anti-HDAC9 antibody (ab18970) 使用論文

This product has been referenced in:
  • Liu J  et al. Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells. J Transl Med 14:7 (2016). Human . Read more (PubMed: 26747087) »
  • Kang I  et al. Ellagic acid inhibits adipocyte differentiation through coactivator-associated arginine methyltransferase 1-mediated chromatin modification. J Nutr Biochem 25:946-53 (2014). ICC/IF ; Human . Read more (PubMed: 24929439) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA with donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Permeabilization Yes - 0.2% Triton X-100 10min
Fixative Paraformaldehyde
Username

Dr. Guoqiang Gu

Verified customer

投稿 Dec 23 2013

I'd suggest trying to use the peptide in excess and incubating the peptide longer with the antibody as well as increasing the incubation volume.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (liver cancer)
Total protein in input 200 µg
Specification liver cancer
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Oct 17 2012

Thank you for your reply.

As requested I am sending you a vial of ab59718 so that you can compare your western blot results to that you get with ab18970. You should receive this antibody tomorrow morning and for your reference the order numbe...

Read More

Thank you for your reply.

Please let me know the catalogue number of the antibody you would like as a replacement. I do not think sending another vial of ab18970 would be helpful, as you are almost guaranteed to get the same results.
Read More

Thank you for your reply.

I will be honest and say I am a little confused as to why you are getting the banding pattern that you are. In human the predicted molecular weight is 110kDa and as you point out in mouse its about 66kDa. I think you...

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Apologies for sending multiply emails none day, but I just heard back from the lab.
It appears we may have incorrect information on the datasheet, as after talking to the lab, the HDAC9 protein comprises of 1011 amino acids with a predicted MW of ...

Read More

Thank you for your patience in this matter. I think the best way forward is for me to send you a free of charge replacement. Any replacement we send will still be covered under our Abpromise (to work as stated on the datasheet).

Please let me...

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Thank you for contacting us.

Blocking will be inefficient if you are mixing the antibody and peptide after first diluting to the working solutions. The better approach is to mix the peptide and antibody stock solutions, then dilute to the wo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (neuroblastoma)
Specification neuroblastoma
Fixative Methanol
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Username

Dr. Bing Lang

Verified customer

投稿 Jan 31 2011

1-10 of 29 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"