製品の概要

  • 製品名Anti-HDAC7 (phospho S318) antibody
    HDAC7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC7 (phospho S318)
  • 特異性Specifically reacts with HDAC7 when phosphorylated at Serine 318. Does not cross-react with non-phosphorylated HDAC7.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Horse, Cow, Dog, Human, Chimpanzee, Monkey, Zebrafish
    交差が予測される動物種: Mouse, Rat, Chicken
  • 免疫原

    A synthetic peptide containing phospho-serine 318 of human HDAC7

  • ポジティブ・コントロール
    • HDAC7 recombinant fusion protein containing a phosphorylated serine at position 318.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 103 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • 細胞内局在Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

Anti-HDAC7 (phospho S318) antibody 画像

  • All lanes : Anti-HDAC7 (phospho S318) antibody (ab72172) at 0.1 µg/ml

    Lane 1 : HDAC7 recombinant fusion protein containing a phosphorylated serine at position 318
    Lane 2 : HDAC7 recombinant fusion protein containing an unphosphorylated serine at position 318


    Predicted band size : 103 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab72172 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72172, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HDAC7 (phospho S318) antibody (ab72172) 使用論文

ab72172 has not yet been referenced specifically in any publications.

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