Anti-HDAC7 抗体 (ab12174)
Key features and details
- Rabbit polyclonal to HDAC7
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-HDAC7 antibody
HDAC7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
KPRLRQIPSAEDLETDG
conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. -
ポジティブ・コントロール
- WB: HeLa and NIH3T3 cell lysates IP: HEK-293T cell lysates
-
特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12174の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. |
|
WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
|
IP |
Use at an assay dependent concentration.
1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7. |
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa. |
IP
Use at an assay dependent concentration. 1.0-1.5 µg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 µg) of 293T cells expressing recombinant human HDAC7. |
ターゲット情報
-
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
ドメイン
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻訳後修飾
May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
細胞内局在
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
-
参照データベース
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
see all -
別名
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
画像
-
All lanes : Anti-HDAC7 antibody (ab12174) at 1/500 dilution
Lane 1 : HeLa nuclear lysate
Lane 2 : NIH3T3 cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 103 kDa -
HDAC7 was immunoprecipitated from whole cell extracts of human HEK-293T cells expressing HDAC7-FLAG using ab12174. The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG® and developed using Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate.
Lane 1: 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of HEK-293T cells expressing HDAC7-FLAG
Lane 2: Negative control (extract without antibody).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (14)
ab12174 は 14 報の論文で使用されています。
- Shen H et al. N6-methyladenosine (m6A) methyltransferase METTL3 regulates sepsis-induced myocardial injury through IGF2BP1/HDAC4 dependent manner. Cell Death Discov 8:322 (2022). WB ; Rat . PubMed: 35840562
- Sato T et al. A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction. Nat Commun 11:3282 (2020). PubMed: 32612176
- Luo Z et al. Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming. Cell Regen (Lond) 8:21-29 (2019). PubMed: 31205685
- Caslini C et al. HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells. Oncogene 38:6599-6614 (2019). PubMed: 31375747
- Wang C et al. Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process. Nat Commun 10:5052 (2019). PubMed: 31699992