製品の概要

  • 製品名
    Anti-HDAC6 (phospho S22) antibody
    HDAC6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC6 (phospho S22)
  • 特異性
    ab61058 detects endogenous levels of HDAC6 only when phosphorylated at serine 22. (Human: Ser22; Mouse: Ser21).
  • アプリケーション
    適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of serine 22 (PQSpPP).

  • ポジティブ・コントロール
    • Human testis tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/5000.

ターゲット情報

  • 機能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
  • 配列類似性
    Belongs to the histone deacetylase family. HD type 2 subfamily.
    Contains 1 UBP-type zinc finger.
  • 翻訳後修飾
    Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
    Sumoylated in vitro.
  • 細胞内局在
    Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CPBHM antibody
    • FLJ16239 antibody
    • HD 6 antibody
    • HD6 antibody
    • HDAC 6 antibody
    • HDAC6 antibody
    • HDAC6_HUMAN antibody
    • Histone deacetylase 6 (HD6) antibody
    • Histone deacetylase 6 antibody
    • JM 21 antibody
    • JM21 antibody
    • KIAA0901 antibody
    • OTTHUMP00000032398 antibody
    • OTTHUMP00000197663 antibody
    • PPP1R90 antibody
    • Protein phosphatase 1 regulatory subunit 90 antibody
    see all

画像

  • Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (phospho S22) antibody (ab61058) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of phosphopeptide.

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Specification
Rat prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jun 29 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP - prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
25 µg
Specification
LNCaP - prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jun 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jun 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (preadipocyte)
Loading amount
4 µg
Specification
preadipocyte
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Hongzhong Wang

Verified customer

投稿 Feb 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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