HDAC6 overexpression 293T lysate (whole cell) (ab94173)

製品の概要

  • 製品名
    HDAC6 overexpression 293T lysate (whole cell)
  • 製品の詳細
    HDAC6 overexpression lysate
  • 特記事項
    ab94173 is a 293T cell transfected lysate in which Human HDAC6 has been transiently over-expressed using a pCMV-HDAC6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. PTM: Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.

アプリケーション

Our Abpromise guarantee covers the use of ab94173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94173 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HDAC6 antibody (ab56926) at 1/500 dilution

    Lane 1 : HDAC6 overexpression 293T lysate (whole cell) (ab94173)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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