製品の概要

  • 製品名Anti-HDAC6 antibody
    HDAC6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC6
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human HDAC6.

    (Peptide available as ab93301.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; HEK293; HUVEC.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
    • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
      Contains 1 UBP-type zinc finger.
    • 翻訳後修飾Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
      Sumoylated in vitro.
    • 細胞内局在Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CPBHM antibody
      • FLJ16239 antibody
      • HD 6 antibody
      • HD6 antibody
      • HDAC 6 antibody
      • HDAC6 antibody
      • HDAC6_HUMAN antibody
      • Histone deacetylase 6 (HD6) antibody
      • Histone deacetylase 6 antibody
      • JM 21 antibody
      • JM21 antibody
      • KIAA0901 antibody
      • OTTHUMP00000032398 antibody
      • OTTHUMP00000197663 antibody
      • PPP1R90 antibody
      • Protein phosphatase 1 regulatory subunit 90 antibody
      see all

    Anti-HDAC6 antibody 画像

    • All lanes : Anti-HDAC6 antibody (ab82557) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 131 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 15 minutes

    Anti-HDAC6 antibody (ab82557) 使用論文

    ab82557 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-12)
    Sample Rat Cell lysate - whole cell (Primary Neurons)
    Specification Primary Neurons
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Jul 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"