製品の概要

  • 製品名Anti-HDAC5 (phospho S498) antibody
    HDAC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC5 (phospho S498)
  • 特異性This antibody is specific for HDAC5, only when phosphorylated at Serine 498.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
    Database link: Q9UQL6

  • ポジティブ・コントロール
    • NIH/3T3 cell extracts; Breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Anti-HDAC5 (phospho S498) antibody 画像

  • All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution

    Lane 1 : Extracts from NIH/ 3T3 cells
    Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)


    Predicted band size : 122 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)
  • ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).

Anti-HDAC5 (phospho S498) antibody (ab47283) 使用論文

This product has been referenced in:
  • Yuan SY  et al. AMPK Mediates Glucocorticoids Stress-Induced Downregulation of the Glucocorticoid Receptor in Cultured Rat Prefrontal Cortical Astrocytes. PLoS One 11:e0159513 (2016). Rat . Read more (PubMed: 27513844) »
  • Mitsi V  et al. RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states. Proc Natl Acad Sci U S A 112:E5088-97 (2015). WB ; Mouse . Read more (PubMed: 26305935) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (5%)
Sample Rat Cell lysate - whole cell (Hippocampal primary Neurons)
Specification Hippocampal primary Neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Nov 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"