Anti-HDAC5 抗体 (ab55403)
Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HDAC5 antibody
HDAC5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC5 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, IHC-FoFr, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
Database link: Q9UQL6 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab55403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55403の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 23469282
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21526203
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 23469282 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21526203 |
WB
1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
ドメイン
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻訳後修飾
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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参照データベース
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
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別名
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
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All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution
Lane 1 : HepG2 cell extract, without immunizing peptide
Lane 2 : HepG2 cell extract, with immunizing peptide
Predicted band size: 122 kDa
Observed band size: 122 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab55403 は 15 報の論文で使用されています。
- Jiang R et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). WB, IHC-P ; Human . PubMed: 35396379
- Duncan HF et al. The Critical Role of MMP13 in Regulating Tooth Development and Reactionary Dentinogenesis Repair Through the Wnt Signaling Pathway. Front Cell Dev Biol 10:883266 (2022). PubMed: 35531096
- Li T et al. HDACs regulate the differentiation of endothelial cells from human iPSCs. Cell Biochem Funct 40:589-599 (2022). PubMed: 35789099
- Ye J et al. HDAC7 Activates IKK/NF-κB Signaling to Regulate Astrocyte-Mediated Inflammation. Mol Neurobiol 59:6141-6157 (2022). PubMed: 35871708
- Azar C et al. RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts. Physiol Rep 9:e14671 (2021). PubMed: 33403800