製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HDAC5
  • 特異性
    ab55403 detects endogenous levels of total HDAC5 protein.
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human HDAC5. Immunogen range: 225-274. Around Serine 259.
    Database link: Q9UQL6

  • ポジティブ・コントロール
    • Human breast carcinoma tissue and HepG2 cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF 1/100. PubMed: 23469282
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

ターゲット情報

  • 機能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメイン
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾
    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 細胞内局在
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.
  • All lanes : Anti-HDAC5 antibody (ab55403) at 1/500 dilution

    Lane 1 : HepG2 cell extract, without immunizing peptide
    Lane 2 : HepG2 cell extract, with immunizing peptide


    Predicted band size : 122 kDa
    Observed band size : 122 kDa

参考文献

This product has been referenced in:
  • Nichols CB  et al. ß-adrenergic signaling inhibits Gq-dependent protein kinase D activation by preventing protein kinase D translocation. Circ Res 114:1398-409 (2014). WB . Read more (PubMed: 24643961) »
  • Takase K  et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . Read more (PubMed: 23469282) »

See all 4 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - BD permeabilization buffer
Sample
Mouse Cell (Mouse thymus tissue, thymocytes)
Specification
Mouse thymus tissue, thymocytes
Gating Strategy
CD45 positives
Preparation
Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

投稿 Jan 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
20 µg
Specification
Mouse brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (cardiomyocyte cell line)
Loading amount
10 µg
Specification
cardiomyocyte cell line
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Paul Townsend

Verified customer

投稿 Oct 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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