製品の概要

  • 製品名Anti-HDAC5 antibody
    HDAC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC5
  • 特異性The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human HDAC5. Synthetic peptide surrounding amino acid 575
    Database link: Q9UQL6

  • ポジティブ・コントロール
    • HeLa or COS cell lysate
  • 特記事項Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 19939859
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 121.9 kDa.

HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • NY CO 9 antibody
    see all

Anti-HDAC5 antibody 画像

  • All lanes : Anti-HDAC5 antibody (ab1439) at 1/1000 dilution

    Lane 1 : Rat hippocampus cell lysate
    Lane 2 : Rat hippocampus cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Polyclonal anti-rabbit IgG HRP conjugate at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121.9 kDa


    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Seungyeon Ko

    See Abreview

  • All lanes : Anti-HDAC5 antibody (ab1439)

    Lane 1 : Cell lysate prepared from HeLa cells
    Lane 2 : Cell lysate prepared from HepG2 cells


    Predicted band size : 121.9 kDa
    Observed band size : 124 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of  HDAC5 in HeLa  (Lane1) and HepG2 (Lane2) cell lysates. A band of approximately 124 KDa can be detected.

Anti-HDAC5 antibody (ab1439) 使用論文

This product has been referenced in:
  • Sharma A  et al. Calcium-mediated histone modifications regulate alternative splicing in cardiomyocytes. Proc Natl Acad Sci U S A 111:E4920-8 (2014). Read more (PubMed: 25368158) »
  • Mielcarek M  et al. SAHA Decreases HDAC 2 and 4 Levels In Vivo and Improves Molecular Phenotypes in the R6/2 Mouse Model of Huntington's Disease. PLoS One 6:e27746 (2011). WB ; Mouse . Read more (PubMed: 22140466) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain cortex)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 min
Permeabilization No
Specification brain cortex
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Abcam user community

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投稿 Jan 30 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Pig Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Oct 08 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (9% gel)
Sample Rat Cell lysate - whole cell (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Apr 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (9% gel)
Sample Mouse Tissue lysate - whole (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

投稿 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Hippocampus)
Loading amount 15 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Seungyeon Ko

Verified customer

投稿 May 14 2013

Application Western blot
Sample Rabbit Cell lysate - whole cell (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (10%SDS)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

投稿 Apr 17 2013

Thank you for contacting us.

SInce this is a polyclonal antibody and not a monoclonal, it will bind to many places on the protein and not to one particular site. Polyclonal antibodies are generally not epitope mapped, and not all of are monocl...

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Thank you for inquiry.

I can confirm that tghe immunogen for ab1439 was a synthetic peptide surrounding amino acid 575 of human HDAC-5.

I also included this information on the datasheet for your information.

I hope this in...

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Thank you for your  reply. I am sending a free of charge vial of ab13939 on the order ***. This product is in stock and will arrive on Monday. Please let me know if you have any questions or if there is anything else that we can do for you.

Thanks for letting me know and keeping me updated. I am sorry to hear that this replacement antibody did not work either. I would be happy to send a different antibody to try or issue a credit or refund. I am running into a meeting but I can gi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"