製品の概要

  • 製品名Anti-HDAC4 antibody - ChIP Grade
    HDAC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC4 - ChIP Grade
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-P, ChIP, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide:

    CISSDPRYWYGKTQHS

    , corresponding to amino acids 194-209 of Human HDAC4

  • ポジティブ・コントロール
    • Lung, respiratory epithelium.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab79521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300. PubMed: 23469282
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 119 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody - ChIP Grade 画像

  • ab79521 at 5µg/ml staining HDAC4 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded lung, respiratory epithelium sections).

Anti-HDAC4 antibody - ChIP Grade (ab79521) 使用論文

This product has been referenced in:
  • Shen W  et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . Read more (PubMed: 24923522) »
  • Takase K  et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . Read more (PubMed: 23469282) »

See all 3 Publications for this product

Product Wall

Je suis ravie que vous souhaitiez participer a notre offre et tester ab79521 en IHC-Fr. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire. Vous devez dans un pre...

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Merci de votre intérêt pour nos anticorps. Comme discuté au téléphone, à ma connaissance, aucun de nos anticorps contre le HDAC4 à été déjà testé sur coupes congélées classiques (IHC-Fr). Si vous souhaitez donc tester ab79521 en IHC-Fr, il me sera poss...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"