製品の概要

  • 製品名Anti-HDAC4 antibody
    HDAC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC4
  • アプリケーション適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4.The corresponding sequence is identical in mouse and differs by one amino acid in chicken.

  • ポジティブ・コントロール
    • Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cells (Western blotting) and HeLa nuclei (immunoprecipitation)
  • 特記事項Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
IP Use 10-20µg for 107 cells.
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • 参照データ���ース
  • 別名
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody (ab12170) 使用論文

ab12170 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample Mouse Cell (Neural cell)
Specification Neural cell
Permeabilization Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative Formaldehyde
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投稿 Oct 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"