The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details.
Use a concentration of 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
Doyle K & Fitzpatrick FA Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem285:17417-24 (2010).
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Imbriano C et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol25:3737-51 (2005).
Read more (PubMed: 15831478) »