製品の概要

  • 製品名Anti-HDAC3 antibody - ChIP Grade
    HDAC3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC3 - ChIP Grade
  • アプリケーション適用あり: IP, ChIP, WBmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    AKTVAYFYDPDVGNFH

    , corresponding to N terminal amino acids 2-17 of Human HDAC3

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution. Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

Anti-HDAC3 antibody - ChIP Grade 画像

  • Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-HDAC3 antibody - ChIP Grade (ab47237) 使用論文

This product has been referenced in:
  • Doyle K & Fitzpatrick FA Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem 285:17417-24 (2010). WB . Read more (PubMed: 20385560) »
  • Imbriano C  et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005). Read more (PubMed: 15831478) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"