製品の概要

  • 製品名Anti-HDAC2 (phospho S394) antibody
    HDAC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC2 (phospho S394)
  • 特異性ab75602 detects endogenous levels of HDAC2 only when phosphorylated at serine 394.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).

  • ポジティブ・コントロール
    • Extracts from HT-29 cells treated with UV (20min). Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 組織特異性Widely expressed; lower levels in brain and lung.
  • 配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 (phospho S394) antibody 画像

  • All lanes : Anti-HDAC2 (phospho S394) antibody (ab75602) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells (untreated)
    Lane 2 : Extracts from HT-29 cells treated with UV (20min)


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75602, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
  • ab75602 at 1/100 dilution, staining HDAC2 (phospho S394) in human Hela cells by ICC/IF.

Anti-HDAC2 (phospho S394) antibody (ab75602) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization Yes - 0.05% tween 20
Specification Lungs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
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投稿 May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"