製品の概要

  • 製品名Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade
    HDAC2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HDAC2-62] to HDAC2 - ChIP Grade
  • アプリケーション適用あり: ChIP, ICC/IF, IHC-Fr, IHC-P, ELISA, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Dog, Human
  • 免疫原

    Synthetic peptide:

    SGEKTDTKGTKSEQLSNP

    with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2.

  • ポジティブ・コントロール
    • Whole cell extracts from NIH-3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration. PubMed: 24919190
ICC/IF 1/50.
IHC-Fr 1/1000. PubMed: 20407577
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 55 kDa.

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 組織特異性Widely expressed; lower levels in brain and lung.
  • 配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade 画像

  • Chromatin was isolated and pooled from the two PF cortices of a single mouse. ChIP experiments were performed using a minimum of 3 mice per group. Chromatin was sheared in an ice bath by a 25 cycles of 30 sec on/off sonication using the “Bioruptor UCD-20” sonicator. Samples were kept on ice during 30 s between two pulses. An aliquot of precleared chromatin was collected as the input. The samples were incubated overnight at 4°C with 5 µl ab12169. The immunoprecipitated chromatin was analyzed by quantitative PCR with primers designed to amplify short regions of the promoters of genes of interest.

    ChIP assay on c-Fos gene promoter using ab12169 antibody. Results show a significant lower enrichment of HDAC 2 in PF cortex of (amyloid precursor protein) APP−/− mouse, Student’s t-test: **p<0.01. IgG was used as negative control. Equal amounts of ChIP and input chromatin were used for qRT-PCR analysis on the c-Fos gene promoter. Enrichment values were normalized to input values and represented the average of three or more mice per experiment. Results are expressed as mean ± SD.

  • ab12169 staining HDAC2 in a human glioblastoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) in PBS and blocked with 0.5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse monoclonal (1/400) was used as the secondary antibody. Nuclei were counterstained with Hoechst (blue).

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  • Western Blot using Rabbit anti-HDAC2

  • All lanes : Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 0.5 µg/ml

    Lane 1 : Hek293T cell Lysate
    Lane 2 : HeLa cell Lysate
    Lane 3 : Jurkat cell Lysate
    Lane 4 : K562 cell Lysate
    Lane 5 : Neuro-2a cell Lysate
    Lane 6 : NIH-3T3 cell Lysate

    Secondary
    Goat Anti-Mouse IgG-Peroxidase
  • Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg

    Secondary
    HRP-conjugated Goat anti-Mouse at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous abreview.

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Anti-HDAC2 antibody [HDAC2-62] - ChIP Grade (ab12169) 使用論文

This product has been referenced in:
  • Lutz L  et al. Histone modifiers and marks define heterogeneous groups of colorectal carcinomas and affect responses to HDAC inhibitors in vitro. Am J Cancer Res 6:664-76 (2016). WB, IHC-P ; Human . Read more (PubMed: 27152243) »
  • Choi SY  et al. Piceatannol Attenuates Renal Fibrosis Induced by Unilateral Ureteral Obstruction via Downregulation of Histone Deacetylase 4/5 or p38-MAPK Signaling. PLoS One 11:e0167340 (2016). Mouse . Read more (PubMed: 27902771) »

See all 20 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human glioblastoma cell line)
Loading amount 120 µg
Specification human glioblastoma cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100 in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (lymphoma, multiple myeloma, prostate, ovarian)
Specification lymphoma, multiple myeloma, prostate, ovarian
Fixative Formaldehyde
Permeabilization Yes - Target Retrieval Solution
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Lena Marquard

Verified customer

投稿 Aug 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (U936 cell line (monocytic))
Specification U936 cell line (monocytic)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Feb 20 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"