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RabMAb

Anti-HDAC2 抗体 [EPR5001] (ab124974)

製品の概要

  • 製品名
    Anti-HDAC2 antibody [EPR5001]
    HDAC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5001] to HDAC2
  • アプリケーション
    適用あり: WB, IP, ICC, ChIPmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N-terminus in Human HDAC2 protein (Q92769).

  • ポジティブ・コントロール
    • WB: HAP1, HDAC2 knockout HAP1, HeLa, A431, SH-SY5Y, and Jurkat cell lysates. ICC/IF: HDAC2 wildtype and knockout HAP1
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
ICC Use a concentration of 0.5 µg/ml.
ChIP Use 10 µg for 25 µg of chromatin.

Please note product formulation is not optimised for ChIP application

  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
    • 組織特異性
      Widely expressed; lower levels in brain and lung.
    • 配列類似性
      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • 翻訳後修飾
      S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • D10Wsu179e antibody
      • HD 2 antibody
      • HD2 antibody
      • HDAC 2 antibody
      • Hdac2 antibody
      • HDAC2_HUMAN antibody
      • Histone deacetylase 2 (HD2) antibody
      • Histone deacetylase 2 antibody
      • OTTHUMP00000017046 antibody
      • OTTHUMP00000227077 antibody
      • OTTHUMP00000227078 antibody
      • RPD3 antibody
      • transcriptional regulator homolog RPD3 antibody
      • YAF1 antibody
      • YY1 associated factor 1 antibody
      • YY1 transcription factor binding protein antibody
      • Yy1bp antibody
      see all

    Anti-HDAC2 antibody [EPR5001] 画像



    • Predicted band size : 55 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: HDAC2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: Hela whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124974 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124974 was shown to recognize HDAC2 when HDAC2 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE.  ab124974 and ab8245 (Mouse anti GAPDH loading control) were but diluted at 1/10000 and incubated overnight at 4°C. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • ab124974 staining HDAC2 in wild-type HAP1 cells (top panel) and HDAC2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124974 at 0.5μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • All lanes : Anti-HDAC2 antibody [EPR5001] (ab124974) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
    • Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 10µg of ab124974 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

    Anti-HDAC2 antibody [EPR5001] (ab124974) 使用論文

    This product has been referenced in:
    • Lin MY  et al. Redirection of Epithelial Immune Responses by Short-Chain Fatty Acids through Inhibition of Histone Deacetylases. Front Immunol 6:554 (2015). WB ; Human . Read more (PubMed: 26579129) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 28 2017

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 25 2015

    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 May 29 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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