Synthetic peptide corresponding to Human HDAC2 aa 471-488 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
Sequence:
C-SGEKTDTKGTKSEQLSNP
Our Abpromise guarantee covers the use of ab7029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/2000 - 1/20000. Predicted molecular weight: 55.3 kDa. | |
IP | Use at an assay dependent concentration. | |
IHC-P | 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. | |
Dot blot | Use at an assay dependent concentration. | |
ICC/IF | Use at an assay dependent concentration. | |
ChIP | Use at an assay dependent concentration. PubMed: 16713578 |
Chromatin was prepared from mouse skin epidermis whole tissue lysate. Cells were fixed with 1% fromaldehyde for 10 minutes. Wild type animal samples were incubated with rabbit IgG or ab7029 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR. Rabbit IgG did not produce significant amplification.
Immunoprecipitation analysis of HeLa whole cell lysates, ab7029 was used to precipitate HDAC3 from solution.
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