製品の概要

  • 製品名Anti-HDAC2 antibody - ChIP Grade
    HDAC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HDAC2 - ChIP Grade
  • 特異性Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kD). The sequence recognized by the antibody is highly conserved in mouse and chicken.
  • アプリケーション適用あり: WB, IP, IHC-P, Dot blot, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken
  • 免疫原

    Synthetic peptide corresponding to Human HDAC2 aa 471-488 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
    Sequence:

    C-SGEKTDTKGTKSEQLSNP

  • ポジティブ・コントロール
    • WB: nuclear extract of HeLa human epithelioid carcinoma cell line. Immunoprecipitation: whole lysate of NIH 3T cells. IHC-P: human lymph node sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/20000. Predicted molecular weight: 55.3 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 16713578

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 組織特異性Widely expressed; lower levels in brain and lung.
  • 配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 antibody - ChIP Grade 画像

  • Immunoprecipitation analysis of HeLa whole cell lysates, ab7029 was used to precipitate HDAC3 from solution.

  • HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/600 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minute
  • Chromatin was prepared from mouse skin epidermis whole tissue lysate.  Cells were fixed with 1% fromaldehyde for 10 minutes.  Wild type animal samples were incubated with rabbit IgG or ab7029 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR.  Rabbit IgG did not produce significant amplification.

    See Abreview

  • HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7029.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: Band: 60ka: HDAC2; 55kDa; Heavy Chain.

Anti-HDAC2 antibody - ChIP Grade (ab7029) 使用論文

This product has been referenced in:
  • Mould AW  et al. Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation. PLoS Genet 11:e1005375 (2015). WB . Read more (PubMed: 26158850) »
  • Xiao X  et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun 6:8266 (2015). WB ; Mouse . Read more (PubMed: 26365427) »

See all 32 Publications for this product

Product Wall

Application Western blot
Sample Monkey Cell lysate - whole cell (Brain)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 12 µg
Specification Brain
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jun 02 2015

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - whole cell (Embryonic Stem Cells)
Specification Embryonic Stem Cells
Negative control Immuno-precipitation with non-specific IgG antibody.
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control WT embryonic stem cells treated with all-trans retinoic acid (1uM)
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投稿 Feb 04 2015

Application Western blot
Loading amount 10000 cells
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - whole cell (huh7 liver)
Specification huh7 liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 26 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample Mouse Cell (Neural cell)
Specification Neural cell
Permeabilization Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative Paraformaldehyde
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投稿 Oct 13 2014

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Application ChIP
Sample Mouse Cell lysate - whole cell (lung carcinoma)
Specification lung carcinoma
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Negative control rabbit IgG
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投稿 Apr 19 2011

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Application Western blot
Sample Mouse Tissue lysate - whole (lymphocyte)
Loading amount 500000 cells
Specification lymphocyte
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 19 2011

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Application ChIP
Sample Human Cell lysate - nuclear (Jurkat Cells)
Specification Jurkat Cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control Anti-Histone 3 antibody from Upstate
Negative control IgG
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投稿 Apr 20 2010

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Application ChIP
Sample Human Cell lysate - whole cell (lymphoblastoid cell line)
Specification lymphoblastoid cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control p21 promoter
Negative control beta actin coding region
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投稿 Mar 24 2010

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Application Western blot
Sample Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount 100000 cells
Specification lymphoblastoid cell line
Treatment shRNA
Gel Running Conditions Non-reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 15 2010

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Application ChIP
Sample Human Cell lysate - nuclear (lung epithelium)
Specification lung epithelium
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Semiquantitative PCR
Negative control rabbit IgG
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投稿 Mar 10 2009

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