製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HDAC11
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HDAC11.

    (Peptide available as ab98895.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate as well as HeLa and NIH3T3 Whole Cell Lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  • 組織特異性
    Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
  • 配列類似性
    Belongs to the histone deacetylase family.
  • 細胞内局在
    Nucleus. Predominantly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ22237 antibody
    • HD 11 antibody
    • HD11 antibody
    • HDA11_HUMAN antibody
    • HDAC 11 antibody
    • HDAC11 antibody
    • Histone deacetylase 11 antibody
    see all

画像

  • All lanes : Anti-HDAC11 antibody (ab87765) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + Recombinant Human HDAC11 protein (ab101113) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 20 minutes

プロトコール

参考文献

ab87765 has not yet been referenced specifically in any publications.

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