Anti-HDAC1 抗体 - ChIP Grade (ab1767)
Key features and details
- Rabbit polyclonal to HDAC1 - ChIP Grade
- Suitable for: WB, ICC/IF, ChIP, IP, IHC - Wholemount
- Reacts with: Drosophila melanogaster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HDAC1 antibody - ChIP Grade
HDAC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HDAC1 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, ChIP, IP, IHC - Wholemountmore details -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide:
C-SGSGTGSGAGAKGAKENNI
, corresponding to amino acids 503-521 of Fruit fly (Drosophila melanogaster) HDAC1 -
特記事項
The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1767の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
|
|
ChIP |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC - Wholemount |
Use at an assay dependent concentration. PubMed: 22195015
|
特記事項 |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC - Wholemount
Use at an assay dependent concentration. PubMed: 22195015 |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
組織特異性
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 38565 Drosophila melanogaster
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別名
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab1767 は 15 報の論文で使用されています。
- Song Z et al. eIF3j inhibits translation of a subset of circular RNAs in eukaryotic cells. Nucleic Acids Res 50:11529-11549 (2022). PubMed: 36330957
- Beaver M et al. Chromatin and transcriptomic profiling uncover dysregulation of the Tip60 HAT/HDAC2 epigenomic landscape in the neurodegenerative brain. Epigenetics N/A:1-22 (2021). PubMed: 34369292
- Beaver M et al. Disruption of Tip60 HAT mediated neural histone acetylation homeostasis is an early common event in neurodegenerative diseases. Sci Rep 10:18265 (2020). PubMed: 33106538
- Panikker P et al. Restoring Tip60 HAT/HDAC2 Balance in the Neurodegenerative Brain Relieves Epigenetic Transcriptional Repression and Reinstates Cognition. J Neurosci 38:4569-4583 (2018). PubMed: 29654189
- Cabezas-Cruz A et al. Anaplasma phagocytophilum increases the levels of histone modifying enzymes to inhibit cell apoptosis and facilitate pathogen infection in the tick vector Ixodes scapularis. Epigenetics 11:303-19 (2016). PubMed: 27019326