製品の概要

  • 製品名Anti-HDAC1 antibody [1.T.9] - ChIP Grade
    HDAC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1.T.9] to HDAC1 - ChIP Grade
  • アプリケーション適用あり: WB, ICC, IP, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Cow, Human, African Green Monkey
    交差が予測される動物種: Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CEEKPEAKGVKEEVKLA

    conjugated to KLH, corresponding to amino acids 467-482 of Mouse HDAC1

  • ポジティブ・コントロール
    • 3T3/A31 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22416134
IHC-FoFr 1/1000. PubMed: 19424149

ターゲット情報

  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 組織特異性Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody [1.T.9] - ChIP Grade 画像

  • ab31263 staining HDAC1 in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA) for 1 hour at 25°C. A DyLight® 488-conjugated goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263) at 0.1 µg/ml + 3T3 cell lysate

    Predicted band size : 60 kDa

Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263) 使用論文

This product has been referenced in:
  • Martínez FP & Tang Q Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem 287:15622-34 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22416134) »
  • Kurita M  et al. HDAC2 regulates atypical antipsychotic responses through the modulation of mGlu2 promoter activity. Nat Neurosci 15:1245-54 (2012). Read more (PubMed: 22864611) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (esophagus)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification esophagus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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投稿 Sep 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Brain)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 12 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 01 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cell (COS7)
Specification COS7
Permeabilization Yes - Triton X100 0.3%
Fixative Paraformaldehyde
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投稿 Oct 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"