製品の概要

  • 製品名Anti-HCV subtype 1b NS5B antibody
    HCV subtype 1b NS5B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HCV subtype 1b NS5B
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Hepatitis C Virus
  • 免疫原

    Recombinant fragment (Hepatitis C Virus) with a 21 amino acid C-terminal truncation

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab35586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 64 kDa.

ターゲット情報

  • 細胞内局在Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • 参照データベース
    • 別名
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Anti-HCV subtype 1b NS5B antibody 画像

    • All lanes : Anti-HCV subtype 1b NS5B antibody (ab35586) at 1/2000 dilution

      Lane 1 : Lysate from Huh7 cells, mock transfection
      Lane 2 : Lysate from Huh7 cells transfected with NS5B


      Observed band size : 64 kDa (why is the actual band size different from the predicted?)

    Anti-HCV subtype 1b NS5B antibody (ab35586) 使用論文

    This product has been referenced in:
    • Carocci M  et al. The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication. Antimicrob Agents Chemother 59:85-95 (2015). Read more (PubMed: 25313218) »
    • Simonin Y  et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog 9:e1003234 (2013). WB . Read more (PubMed: 23555249) »

    See all 10 Publications for this product

    Product Wall

    Thank you for contacting us.

    The lab sent me the correct immunogen info:
    "Recombinant HCV (Hepatitis C Virus) NS5B with a 21 amino acid C-terminal truncation, genotype 1b, clone Con1, UniProt link
    http://www.uniprot.org/uniprot/?query...

    Read More

    Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge z...

    Read More

    Vielen Dank dafür, dass Sie nachhaken.

    Leider haben wir noch keine guten Nachrichten für Sie:

    Wie versprochen haben wir uns in den entsprechenden Laboren erkundigt. Bislang haben wir für unsere anti-HCV NS5B-Antik&oum...

    Read More

    Thank you for contacting us. I am sorry for the delay in this response. ab100895 is a Mouse monoclonal antibody to Hepatitis C virus NS5B which has been used previously for ELISA and Western blotting. Unfortunately I have been unable to confirm if this...

    Read More
    Application ELISA
    Sample Human Cell (huh-7 hepatocarcinoma cells)
    Specification huh-7 hepatocarcinoma cells
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    投稿 Jun 17 2008

    Thank you for your enquiry. I can confirm that this antibody works well with both 1a and 1b genotypes and also should work with many other genotypes as it is a polyclonal raised to an almost full-length protein. I hope this information is helpf...

    Read More

    Thank you for your patience. Regarding ab35586 (Hepatitis C virus NS5B antibody), this antibody will recognize genotype 1a. Please don't hesitate to contact us again if you have further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"