Anti-HCV subtype 1b NS5B 抗体 [10D6] (ab100895)
Key features and details
- Mouse monoclonal [10D6] to HCV subtype 1b NS5B
- Suitable for: WB, IP, ELISA
- Reacts with: Hepatitis C virus
- Isotype: IgG1
製品の概要
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製品名
Anti-HCV subtype 1b NS5B antibody [10D6]
HCV subtype 1b NS5B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10D6] to HCV subtype 1b NS5B -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ELISAmore details -
種交差性
交差種: Hepatitis C virus -
免疫原
Recombinant fragment:
LSSKAVNHIRSVWKDLLEDT
, corresponding to amino acids 111-130 of HCV subtype 1b NS5B -
ポジティブ・コントロール
- Huh7 cells harboring selectable subgenomic HCV RNA replicon
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
10D6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100895の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 65 kDa.
|
IP |
Use at an assay dependent dilution.
|
|
ELISA |
1/1000.
|
特記事項 |
---|
WB
1/1000. Predicted molecular weight: 65 kDa. |
IP
Use at an assay dependent dilution. |
ELISA
1/1000. |
ターゲット情報
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細胞内局在
Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted. -
参照データベース
- SwissProt: Q9WMX2 Hepatitis C virus
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別名
- Hepacivirin antibody
- NS3P antibody
- POLG_HCVBK antibody
- Serine protease NS3 antibody
画像
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IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
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All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
Lane 1 : Huh-7
transfected with HCV replicon
Lane 2 : Huh-7 nontransfected
cells
Predicted band size: 65 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100895 は 2 報の論文で使用されています。
- Chen SR et al. In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn. Molecules 21:N/A (2016). PubMed: 27754461
- Vogt DA et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . PubMed: 23593007