製品の概要

  • 製品名Anti-HCP1 antibody
    HCP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HCP1
  • 特異性The immunogen used to raise this antibody has 80% homology with the human HCP1 protein. Some customers have successfully used ab25134 in human tissue. Please contact Abcam Scientific Support for more information.
  • アプリケーション適用あり: IHC-P, ELISA, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse HCP1.

    (Peptide available as ab25847.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in IF the following Formaldehyde fixed cell lines: NIH3T3. In Western Blot, this antibody gave a positive signal in NIH3T3 whole cell lysate and a positive signal in the following tissue lysates: Rat Liver; Rat Spleen; Rat Heart; Mouse Kidney.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.
  • 組織特異性Expressed in kidney, liver, placenta, small intestine, spleen, retina and retinal pigment epithelium. Lower levels found in colon and testis. Very low levels in brain, lung, stomach, heart and muscle. In intestine, expressed in duodenum with lower levels in jejunum, ileum, cecum, rectum and segments of the colon.
  • 関連疾患Defects in SLC46A1 are the cause of hereditary folate malabsorption (HFM) [MIM:229050]. HFM is a rare autosomal recessive disorder characterized by impaired intestinal folate absorption with folate deficiency resulting in anemia, hypoimmunoglobulinemia with recurrent infections, and recurrent or chronic diarrhea. In many patients, neurological abnormalities such as seizures or mental retardation become apparent during early childhood, attributed to impaired transport of folates into the central nervous system. When diagnosed early, the disorder can be treated by administration of folate. If untreated, it can be fatal and, if treatment is delayed, the neurological defects can become permanent.
  • 配列類似性Belongs to the major facilitator superfamily. SLC46A family.
  • 細胞内局在Apical cell membrane. Cytoplasm. Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in the cytoplasm in iron-replete cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G21 antibody
    • HCP 1 antibody
    • Heme carrier protein 1 antibody
    • MGC9564 antibody
    • PCFT antibody
    • PCFT/HCP1 antibody
    • PCFT_HUMAN antibody
    • PDE7A antibody
    • Proton coupled folate transporter antibody
    • Proton-coupled folate transporter antibody
    • SLC46A1 antibody
    • Solute carrier family 46 (folate transporter) member 1 antibody
    • Solute carrier family 46 member 1 antibody
    see all

Anti-HCP1 antibody 画像

  • All lanes : Anti-HCP1 antibody (ab25134) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Spleen (Rat) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate
    Lane 4 : Kidney (Mouse) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • All lanes : Anti-HCP1 antibody (ab25134) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ICC/IF image of ab25134 stained NIH-3T3 cells. The cells were 4% paraformladhyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25134, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Image courtesy of Human Protein Atlas

    ab25134 staining HCP1 in human stomach. Paraffin embedded human skin tissue was incubated with ab25134 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab25134 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

Anti-HCP1 antibody (ab25134) 使用論文

This product has been referenced in:
  • Takada T  et al. Selective accumulation of hematoporphyrin derivative in glioma through proton-coupled folate transporter SLC46A1. J Clin Biochem Nutr 54:26-30 (2014). IHC . Read more (PubMed: 24426187) »
  • Cao C  et al. Duodenal absorption and tissue utilization of dietary heme and nonheme iron differ in rats. J Nutr 144:1710-7 (2014). Read more (PubMed: 25332470) »

See all 6 Publications for this product

Product Wall


I have heard back from the lab with the following explanation regarding the membrane vs. cytoplasmic staining:
From what we can gather this protein can also show cytoplasmic localisation (swissprot).
It's very difficult to distinguish betw...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your reply.

A free of charge vial of ab98043 has been issued, which should arrive tomorrow.

To check the status of the order please contact our Customer Service team and reference this number.

Please note ...

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DISCOUNT CODE: 100%000000

Expiration date: 14. October 2012


I am very pleased to hear you would like to accept our offer and test ab25134 in flow cytometry. This code will give you the value of ab25134 off a futuire order bef...

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Thank you very much for your interest in ab25134.

To our knowledge,ab25134 has not been tested in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab25134 now, test it inflow cytometry and submit feedback to...

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for following up with your results.

I am sorry that the blocking peptide experiments did not demonstrate the specificity of the bands you were detecting in your samples. I have checked our available stock and we only have the lot y...

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Thank you for your reply.


The new results you obtained are quite interesting. The image on our website is not representing data from the current lot, but is representative of the expected results with this antibody.


Per ...

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Thank you for contacting Abcam regarding ab25134.


I have asked the lab to retest this antibody. We observed an ˜60 kDa in most Hu tissues and an ˜50 kDa in Ms and Rat tissues, consistent with our ds images. We used 5% BSA to block so it...

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We have previously been in contact regarding ab25134, which was not providing satisfactory results.

I would appreciate if you could let me know whether satisfactory results were obtained with the antibody.

I wish you the best of l...

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1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"