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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse HCP1.
(Peptide available as ab25847.)
Our Abpromise guarantee covers the use of ab25134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).|
Image courtesy of Human Protein Atlas
ab25134 staining HCP1 in human stomach. Paraffin embedded human skin tissue was incubated with ab25134 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab25134 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org