製品の概要

  • 製品名Anti-HCN2 antibody [S71-37]
    HCN2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S71-37] to HCN2
  • 特異性Does not cross react with HCN1.
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 761-863 of Rat HCN2

  • ポジティブ・コントロール
    • Rat brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • 組織特異性Highly expressed throughout the brain. Detected at low levels in heart.
  • 配列類似性Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • ドメインThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Anti-HCN2 antibody [S71-37] 画像

  • Anti-HCN2 antibody [S71-37] (ab84817) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size : 95 kDa
  • ab84817 staining HCN2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • ab84817 staining HCN2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

Anti-HCN2 antibody [S71-37] (ab84817) 使用論文

ab84817 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HL-1 cardiac cell line)
Specification HL-1 cardiac cell line
Fixative Methanol
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"