製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HCLS1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: QRWGAKTIEG SGRTEHINIH QLRNKVSEEH DVLRKKEMES GPKASHGYGG, corresponding to N terminal amino acids 36-85 of Human HCLS1

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • 組織特異性
    Expressed only in tissues and cells of hematopoietic origin.
  • 配列類似性
    Contains 4 cortactin repeats.
    Contains 1 SH3 domain.
  • 発生段階
    Expressed in early stage of myeloid and erythroid differentiation.
  • 翻訳後修飾
    Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.
  • 細胞内局在
    Membrane. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cortactin like antibody
    • CTTNL antibody
    • HCLS 1 antibody
    • Hcls1 antibody
    • HCLS1_HUMAN antibody
    • Hematopoietic cell specific Lyn substrate 1 antibody
    • Hematopoietic cell-specific LYN substrate 1 antibody
    • Hematopoietic lineage cell-specific protein antibody
    • HS 1 antibody
    • HS1 antibody
    • LckBP1 antibody
    • OTTHUMP00000215180 antibody
    • OTTHUMP00000215182 antibody
    • p75 antibody
    see all

画像

  • Anti-HCLS1 antibody (ab28725) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Ab28725, at a concentration of 4 - 8 µg/ml, staining HCLS1 in paraffin embedded human kidney tissue by Immunohistochemistry. Cells with positive label (as indicated by the arrows) are epithelial cells of the renal tubule. Magnification: 400X.
  • Immunohistochemistry of Human Heart lysate tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

  • Immunohistochemistry of Human Stomach tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

参考文献

ab28725 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. The bands in molecular weight marker we used before are a little deviate from the expected locations, therefore the 60kD band is about 70kD according to our new calibrated marker. This product should detect a band at arou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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