製品の概要

  • 製品名Anti-HCLS1 antibody
    HCLS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HCLS1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: QRWGAKTIEG SGRTEHINIH QLRNKVSEEH DVLRKKEMES GPKASHGYGG, corresponding to N terminal amino acids 36-85 of Human HCLS1

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab28725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • 組織特異性Expressed only in tissues and cells of hematopoietic origin.
  • 配列類似性Contains 4 cortactin repeats.
    Contains 1 SH3 domain.
  • 発生段階Expressed in early stage of myeloid and erythroid differentiation.
  • 翻訳後修飾Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.
  • 細胞内局在Membrane. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cortactin like antibody
    • CTTNL antibody
    • HCLS 1 antibody
    • Hcls1 antibody
    • HCLS1_HUMAN antibody
    • Hematopoietic cell specific Lyn substrate 1 antibody
    • Hematopoietic cell-specific LYN substrate 1 antibody
    • Hematopoietic lineage cell-specific protein antibody
    • HS 1 antibody
    • HS1 antibody
    • LckBP1 antibody
    • OTTHUMP00000215180 antibody
    • OTTHUMP00000215182 antibody
    • p75 antibody
    see all

Anti-HCLS1 antibody 画像

  • Anti-HCLS1 antibody (ab28725) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Ab28725, at a concentration of 4 - 8 µg/ml, staining HCLS1 in paraffin embedded human kidney tissue by Immunohistochemistry. Cells with positive label (as indicated by the arrows) are epithelial cells of the renal tubule. Magnification: 400X.
  • Immunohistochemistry of Human Heart lysate tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

  • Immunohistochemistry of Human Stomach tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

Anti-HCLS1 antibody (ab28725) 使用論文

ab28725 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The bands in molecular weight marker we used before are a little deviate from the expected locations, therefore the 60kD band is about 70kD according to our new calibrated marker. This product should detect a band at arou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"