Anti-Hck (phospho Y410) 抗体 (ab61055)

製品の概要

  • 製品名
    Anti-Hck (phospho Y410) antibody
    Hck 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hck (phospho Y410)
  • 特異性
    Detects target only when phosphorylated at tyrosine 410.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)

  • ポジティブ・コントロール
    • HeLa cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.

ターゲット情報

  • 機能
    May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • 組織特異性
    Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • ドメイン
    The SH3 domain mediates binding to HIV-1 Nef.
  • 細胞内局在
    Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名
    • Bmk antibody
    • Hck 1 antibody
    • Hck antibody
    • HCK_HUMAN antibody
    • Hemopoietic cell kinase antibody
    • JTK9 antibody
    • p59-HCK/p60-HCK antibody
    • p59Hck antibody
    • p59HCK/p60HCK antibody
    • p61Hck antibody
    • Tyrosine protein kinase HCK antibody
    • Tyrosine-protein kinase HCK antibody
    see all

画像

  • ab61055 staining Hck (phospho Y410) in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour; antigen retrieval was by heat mediation in trypsin. Samples were incubated with primary antibody (1/100) for 24 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • 1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

参考文献

ab61055 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trypsin
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
10% Formalin
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投稿 Oct 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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