RabMAb

Anti-Hck 抗体 [EP2415Y] (ab75839)

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製品の概要

  • 製品名
    Anti-Hck antibody [EP2415Y]
    Hck 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2415Y] to Hck
  • 由来種
    Rabbit
  • 特異性
    This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.
  • アプリケーション
    適用あり: WB, IP, ICC, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human Hck (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール
    • TF-1 cell lysate.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000 - 1/200000. Predicted molecular weight: 59 kDa.
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for IHC-P.

    • ターゲット情報

    • 機能
      May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
    • 組織特異性
      Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • ドメイン
      The SH3 domain mediates binding to HIV-1 Nef.
    • 細胞内局在
      Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
    • Information by UniProt
    • 参照データベース
      • 製品の状態
        This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
      • 別名
        • Bmk antibody
        • Hck 1 antibody
        • Hck antibody
        • HCK_HUMAN antibody
        • Hemopoietic cell kinase antibody
        • JTK9 antibody
        • p59-HCK/p60-HCK antibody
        • p59Hck antibody
        • p59HCK/p60HCK antibody
        • p61Hck antibody
        • Tyrosine protein kinase HCK antibody
        • Tyrosine-protein kinase HCK antibody
        see all

      画像

      • Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        Western blot - Anti-Hck antibody [EP2415Y] (ab75839)
        Anti-Hck antibody [EP2415Y] (ab75839) at 1/200000 dilution + TF-1 cell lysate at 10 µg

        Secondary
        goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 59 kDa
        Observed band size: 59 kDa

      参考文献

      ab75839 has not yet been referenced specifically in any publications.

      ab75839 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      1 Review or Q&A

      10
      Votes: Highest First

      This antibody (cat #2254-1) looks highly interesting. However, as the C-terminal region of the protein shares homology with other SRC-related tyrosine kinases, I wonder whether specificity against HCK vs SRC, LCK, YES, LYN, FYN, FGR and BLK has been te...

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      We have not tested ab75839 in-house for cross reactivity with LCK,YES, or BLK so we cannot say for certain whether this antibody would be suitable for detecting these targets. However, in our WB tests we have only observed a single band at ~ 60 KDa wh...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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