The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Use at an assay dependent dilution. Reported to Immunoprecipitate Hck from 1-2 x 10 7 THP-1 monocytic cells lysed in RIPA.
機能May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
組織特異性Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
ドメインThe SH3 domain mediates binding to HIV-1 Nef.
細胞内局在Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
製品の状態This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
Hck 1 antibody
Hemopoietic cell kinase antibody
Tyrosine protein kinase HCK antibody
Tyrosine-protein kinase HCK antibody
Anti-Hck antibody (ab32860) 使用論文
This product has been referenced in:
Zhu L et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med12:1181-1184 (2016).
Read more (PubMed: 27446340) »
Xiao X et al. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am J Physiol Endocrinol Metab304:E145-59 (2013).
Read more (PubMed: 23169788) »