The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-P: Used at a dilution of 1/1500 for 2 hrs on human placenta sections (see Abreview for further details).
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution (see Berger et al).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Human chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, by the developing placenta and by the fertilized egg after implantation in the uterine wall. Its primary role is in the maintenance of the corpus luteum and causes it to secrete the hormone progesterone. Progesterone enriches the uterus with a thick lining of blood vessels and capillaries so that it can sustain the growing fetus. HCG is composed of two subunits, alpha and beta. The alpha subunit of HCG, however, is identical to the alpha subunit of luteinising hormone, thyroid stimulating hormone and follicle stimulating hormone.
Serum human chorionic gonadotrophin testing is accurate 48 hour post conception. Urine human chorionic gonadotrophin testing is a popular method of home pregnancy determination as human chorionic gonadotrophin can be detected in urine
Forbes K et al. Insulin-like growth factor I and II regulate the life cycle of trophoblast in the developing human placenta. Am J Physiol Cell Physiol294:C1313-22 (2008).
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