Anti-HCF-1 / Host Cell Factor C1 抗体 (ab94631)

製品の概要

  • 製品名Anti-HCF-1 / Host Cell Factor C1 antibody
    HCF-1 / Host Cell Factor C1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HCF-1 / Host Cell Factor C1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1986-2035 (RNEKGYGPAT QVRWLQETSK DSSGTKPANK RPMSSPEMKS APKKSKADGQ) of Human Host Cell Factor C1 (NP_005325).

  • ポジティブ・コントロール
    • Human fetal stomach lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 209 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
  • 組織特異性Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
  • 配列類似性Contains 5 Kelch repeats.
  • ドメインThe HCF repeat is a highly specific proteolytic cleavage signal.
    The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.
  • 翻訳後修飾Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
    O-glycosylated.
    Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.
  • 細胞内局在Cytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1 factor antibody
    • CFF antibody
    • HCF 1 antibody
    • HCF antibody
    • HCF C-terminal chain 6 antibody
    • HCF-1 antibody
    • HCF1 antibody
    • Hcfc1 antibody
    • HCFC1_HUMAN antibody
    • HFC1 antibody
    • Host cell factor 1 antibody
    • Host cell factor antibody
    • Host cell factor C1 (VP16 accessory protein) antibody
    • Host cell factor C1 antibody
    • MGC70925 antibody
    • MRX3 antibody
    • VCAF antibody
    • VP16 accessory protein antibody
    see all

Anti-HCF-1 / Host Cell Factor C1 antibody 画像



  • Predicted band size : 209 kDa
    Western blot analysis of HIS-HCFC1 16-363aa (lane 1 - 42kD) transformed bacteria lysate and GFP-HCFC1 363-2002aa (73kD - lane 2) transformed bacteria lysate elution sample labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1:1000. An Alexa Fluor 680-conjugated anti-rabbit (1:10000) was used as the secondary antibody.


  • Predicted band size : 209 kDa
    Western blot analysis of Human fetal liver tissue lsyate labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1.0µg/ml.


  • Predicted band size : 209 kDa
    Western blot analysis of Human fetal heart tissue lsyate labeling HCF-1 / Host Cell Factor C1 with ab94631 at 1.0µg/ml.
  • Anti-HCF-1 / Host Cell Factor C1 antibody (ab94631) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 209 kDa
    Gel concentration: 6-18%

Anti-HCF-1 / Host Cell Factor C1 antibody (ab94631) 使用論文

ab94631 has not yet been referenced specifically in any publications.

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