製品の概要

  • 製品名Anti-HCE antibody
    HCE 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to HCE
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) with 6x His tag

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  • 組織特異性Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
  • 配列類似性In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
    In the C-terminal section; belongs to the eukaryotic GTase family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAP1A antibody
    • GTase antibody
    • GTP--RNA guanylyltransferase antibody
    • hCAP antibody
    • HCAP1 antibody
    • HcE antibody
    • HCE1 antibody
    • MCE1 antibody
    • MCE1_HUMAN antibody
    • mRNA 5''-triphosphatase antibody
    • mRNA capping enzyme antibody
    • mRNA guanyltransferase antibody
    • mRNA guanylyltransferase antibody
    • Polynucleotide 5' trisphosphatase antibody
    • RNA guanylyltransferase and 5' phosphatase antibody
    • RNGTT antibody
    • TPase antibody
    see all

Anti-HCE antibody 画像

  • All lanes : Anti-HCE antibody (ab27971) at 1/1000 dilution

    Lane 1 : Hela nuclear extract at 0.5 µl
    Lane 2 : Hela nuclear extract at 1 µl

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa

Anti-HCE antibody (ab27971) 使用論文

This product has been referenced in:
  • Adamson TE  et al. Functional coupling of cleavage and polyadenylation with transcription of mRNA. J Biol Chem 280:32262-71 (2005). Read more (PubMed: 16041059) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"