The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa). An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.
Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.
ab115013 (5µg/ml) shows staining of HCCA2 in paraffin embedded Human Lung following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole.
Western blot - Anti-HCCA2 antibody (ab115013)
Anti-HCCA2 antibody (ab115013) at 0.02 µg/ml + Jurkat cell lysate at 35 µg